The morbidity and mortality of methicillin-resistant Staphylococcus aureus (MRSA) bloodstream infection remain high. The positive blood culture of MRSA is the gold standard for diagnosis of bacteremia. After clinical diagnosis of MRSA bloodstream infection, clinicians should identify the possible primary infection sources and dissemination sites. This is the premise and foundation of successful and timely treatment of MRSA bloodstream infection. The guidelines have recommended Vancomycin and Daptomycin as first-line antibiotics in the treatment of MRSA bloodstream infections, while Linazolamide can be used as a salvage therapy for patients with vancomycin resistance or renal dysfunction. Remarkably, it is of great significance to systematically review some hot issues of MRSA bloodstream infection, with an emphasis on the sources of infection and dissemination sites.