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中华重症医学电子杂志 ›› 2024, Vol. 10 ›› Issue (03) : 248 -252. doi: 10.3877/cma.j.issn.2096-1537.2024.03.007

综述

单细胞测序在急性呼吸窘迫综合征中的应用进展
杨永红1, 杨莹1, 齐红蕾1, 刘福瑞1, 朱金源2,()   
  1. 1. 750004 宁夏,宁夏医科大学第一临床医学院
    2. 750004 宁夏,宁夏医科大学总医院重症医学科
  • 收稿日期:2024-02-03 出版日期:2024-08-28
  • 通信作者: 朱金源
  • 基金资助:
    国家自然科学基金项目(82360022); 宁夏重点研发计划项目(2022BEG03102); 宁夏自然科学基金项目(2022AAC03466)

Application of single-cell sequencing in acute respiratory distress syndrome

Yonghong Yang1, Ying Yang1, Honglei Qi1, Furui Liu1, Jinyuan Zhu2,()   

  1. 1. College of First Clinical Medical, Ningxia Medical University, Yinchuan 750004, China
    2. Department of Intensive Care Medicine, General Hospital of Ningxia Medical University, Yinchuan 750004, China
  • Received:2024-02-03 Published:2024-08-28
  • Corresponding author: Jinyuan Zhu
引用本文:

杨永红, 杨莹, 齐红蕾, 刘福瑞, 朱金源. 单细胞测序在急性呼吸窘迫综合征中的应用进展[J]. 中华重症医学电子杂志, 2024, 10(03): 248-252.

Yonghong Yang, Ying Yang, Honglei Qi, Furui Liu, Jinyuan Zhu. Application of single-cell sequencing in acute respiratory distress syndrome[J]. Chinese Journal of Critical Care & Intensive Care Medicine(Electronic Edition), 2024, 10(03): 248-252.

急性呼吸窘迫综合征(ARDS)是由肺内和肺外因素所致的弥漫性炎性肺泡及肺毛细血管损伤为特征的临床综合征。ARDS发病机制复杂,细胞间普遍存在异质性,传统测序技术是以大量细胞或组织为研究样本,反映的是细胞总体上转录组特征,无法体现生物细胞亚群多样性和个体细胞的异质性,限制了疾病潜在靶点的发掘和应用。单细胞测序为深入研究ARDS单个细胞的独特基因表达模式和分子机制提供了新的手段,对了解ARDS的发病机制、临床特征、筛选潜在治疗靶点,具有重要意义。该综述为单细胞测序在ARDS中的应用提供了更多依据。

Acute respiratory distress syndrome (ARDS) is a clinical syndrome characterized by diffuse inflammatory alveolar and pulmonary capillary injury caused by both pulmonary and extrapulmonary factors. Due to the complexity of the pathogenesis and widespread heterogeneity at the cellular level, traditional sequencing techniques, which use many cells or tissues as study samples, reflect the overall transcriptomic characteristics of cells but cannot capture the diversity of cellular subgroups and individual cell heterogeneity. This limitation hinders the exploration and application of potential disease targets. Single-cell sequencing provides a new approach to investigate the unique gene expression patterns and molecular mechanisms of individual cells in ARDS. It is crucial for understanding the pathogenesis, clinical features, and identification of potential therapeutic targets in ARDS. This review offers additional insights into the application of single cell sequencing in ARDS, providing a more solid foundation for research in this field.

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